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Safety Data Sheet EN

Safranin, 1% Alcoholic

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01

Identification

Product identifiers, regulatory numbers, and supplier information

Product Information

Product Name
Safranin, 1% Alcoholic
Product Form
Mixture
Product Code
LC22360

Regulatory Identifiers

CAS Number
N/A

Identified Uses

For laboratory and manufacturing use only.

Manufacturers & Suppliers

LabChem Inc logo

LabChem Inc

manufacturer

Jackson's Pointe Commerce Park Building 1000, 1010 Jackson's Pointe Court, Zelienople, PA 16063 - USA

412-826-5230

http://www.labchem.com

Emergency Contacts

CHEMTREC

1-800-424-9300

CHEMTREC

011-703-527-3887

02

Hazard Identification

GHS classification, signal word, pictograms, and hazard statements

Classified danger

Hazard Classifications

Flam. Liq. Cat. 2 H225
Eye Irrit. Cat. 2A H319
Carc. Cat. 1A H350
Repr. Cat. 2 H361
STOT SE Cat. 1 H370

GHS Pictograms

GHS02 - Flammable

GHS02

GHS07 - Health hazard

GHS07

GHS08 - Serious health hazard

GHS08

Hazard Statements

H225 Highly flammable liquid and vapor
H319 Causes serious eye irritation
H350 May cause cancer
H361 Suspected of damaging fertility or the unborn child
H370 Causes damage to organs (central nervous system, optic nerve, liver, kidneys)

Precautionary Statements

P201 Obtain special instructions before use
P202 Do not handle until all safety precautions have been read and understood
P210 Keep away from heat, sparks, open flames, hot surfaces. - No smoking
P233 Keep container tightly closed
P240 Ground/bond container and receiving equipment
P241 Use explosion-proof electrical, ventilating, lighting equipment
P242 Use only non-sparking tools
P243 Take precautionary measures against static discharge
P260 Do not breathe mist, vapors, spray
P264 Wash exposed skin thoroughly after handling
P270 Do not eat, drink or smoke when using this product
P280 Wear protective gloves, eye protection
P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower
P305 + P351 + P338 If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
P308 + P313 IF exposed or concerned: Get medical advice/attention
P337 + P313 If eye irritation persists: Get medical advice/attention
P370 + P378 In case of fire: Use alcohol resistant foam, carbon dioxide (CO2), extinguishing powder to extinguish
P403 + P235 Store in a well-ventilated place. Keep cool
P405 Store locked up
P501 Dispose of contents/container to comply with local, state and federal regulations

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03

Composition / Information on Ingredients

Chemical components, concentration ranges, and hazardous substance identification

Type mixture
Chemical Name CAS Number Concentration Hazardous
Ethanol 64-17-5 89.1% Yes
Methanol 67-56-1 4.95% Yes
Isopropanol 67-63-0 4.95% Yes
Safranin O 477-73-6 1% Yes
04

First Aid Measures

Emergency procedures for chemical exposure incidents

Inhalation

Remove victim to fresh air and keep at rest in a position comfortable for breathing.

Symptoms: Dizziness. Headache.

Skin contact

Rinse skin with water/shower. Remove/Take off immediately all contaminated clothing.

Symptoms: May stain the skin. Visual disturbances.

Eye contact

Remove contact lenses, if present and easy to do. Continue rinsing. Rinse cautiously with water for several minutes. Obtain medical attention if pain, blinking or redness persist.

Symptoms: May cause irritation.

Ingestion

Rinse mouth. Do NOT induce vomiting. Obtain emergency medical attention. Call a POISON CENTER or doctor/physician if you feel unwell.

Symptoms: Swallowing a small quantity of this material will result in serious health hazard. Blindness. Visual disturbances. Vomiting. Nausea. Dizziness. Drunkenness. Central nervous system depression.

Immediate Medical Attention

Obtain medical assistance.

05

Firefighting Measures

Extinguishing media, specific hazards, and firefighter protection

Suitable media

Foam. Dry powder. Carbon dioxide. Water spray. Sand.

Unsuitable media

Do not use a heavy water stream.

Specific hazards

Highly flammable liquid and vapor. May form flammable/explosive vapor-air mixture.

Instructions

Use water spray or fog for cooling exposed containers. Exercise caution when fighting any chemical fire.

Firefighter Protection

Do not enter fire area without proper protective equipment, including respiratory protection.

06

Accidental Release Measures

Spill cleanup procedures, containment, and environmental protection

Emergency procedures

Evacuate unnecessary personnel. Ventilate area.

Environmental

Prevent entry to sewers and public waters.

Cleanup methods

Soak up spills with inert solids, such as clay or diatomaceous earth as soon as possible. Collect spillage. Store away from other materials.

Materials: inert solids, such as clay or diatomaceous earth

—

Related Products

Similar products with comparable safety profiles

S

Safranin

---

S

Safranin O

CAS: 477-73-6

S

Safranin O

CAS: 477-73-6

S

Safranin T

CAS: 477-73-6

S

Safranine O

CAS: 477-73-6

S

Safranine O (C.I. 50240) for microscopy Certistain®

---

E

Enhanced Gram Safranin

---

G

Gram's safranine solution for the Gram staining method

---

07

Handling and Storage

Safe handling precautions, storage conditions, and workplace requirements

Handling

Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work. Provide good ventilation in process area to prevent formation of vapor. No naked lights. No smoking. Use only non-sparking tools. Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Do not breathe mist, vapors, spray.

Storage

Keep only in the original container in a cool, well ventilated place away from : Heat sources., Ignition sources, incompatible materials. Keep in fireproof place. Keep container tightly closed.

Hygiene

Do not eat, drink or smoke when using this product. Wash exposed skin thoroughly after handling.

Fire prevention

No naked lights. No smoking. Use only non-sparking tools.

08

Exposure Controls / PPE

Occupational exposure limits, engineering controls, and protective equipment

Engineering

Emergency eye wash fountains and safety showers should be available in the immediate vicinity of any potential exposure. Ensure adequate ventilation. Material should be handled in a laboratory hood whenever possible.

Hands

Wear protective gloves.

Eyes

Chemical goggles or safety glasses.

Respiratory

Where exposure through inhalation may occur from use, respiratory protection equipment is recommended.

Skin/Body

Chemical resistant apron.

09

Physical and Chemical Properties

Appearance, physical state, melting point, boiling point, and material characteristics

Language Code
---
Physical State
Liquid
Physical State Data
liquid
State Under Standard Conditions
---
Appearance
---
Colour
red
Colour Intensity
---
Form
---
Odor
Alcohol odour
Odor Threshold
100 ppm 188 mg/m³
Ph
---
Melting Point
-115 °C
Boiling Point
78 °C
Flash Point
13 °C
Freezing Point
---
Softening Point
---
Solidification Point
---
Cloud Point
---
Crystallisation Point
---
Relative Evaporation Rate
2.4 (butyl acetate=1); 8.3 (ether=1)
Evaporation Rate
---
Vapor Pressure
59 hPa
Vapor Density
---
Relative Density
0.79
Bulk Density
---
Density
---
Flammability
---
Upper Explosive Limit
19 vol %
Lower Explosive Limit
3.3 vol %
Explosive Limits
---
Auto Ignition Temperature
363 °C
Decomposition Temperature
---
Solubility
Soluble in water. Soluble in methanol. Soluble in isopropanol. Soluble in ethanol. Water: Solubility in water of component(s) of the mixture : • Ethanol: • Methanol: >= 100 g/100ml • Isopropanol:
Partition Coefficient
---
Solubility In Water
---
Solubility In Fat
---
Molecular Weight
---
Voc Content
---
Metal Corrosion
---
Dynamic Viscosity
0.0012 Pa.s (20°C)
Kinematic Viscosity
---
Properties Status
---
10

Stability and Reactivity

Chemical stability, hazardous reactions, and incompatible materials

Stability

Highly flammable liquid and vapor. May form flammable/explosive vapor-air mixture.

Reactivity

No additional information available

Hazardous reactions

Not established.

Avoid

Direct sunlight. Extremely high or low temperatures. Open flame.

Incompatible

Strong acids. Strong bases. Strong oxidizers.

Decomposition

Carbon monoxide. Carbon dioxide. Nitrogen oxides.

12

Ecological Information

Environmental toxicity, biodegradation, and bioaccumulation data

12.1. Toxicity

| Ethanol (64-17-5) | | | --- | --- | | LC50 fish 1 | 14200 mg/l (96 h; Pimephales promelas) | | EC50 Daphnia 1 | 9300 mg/l (48 h; Daphnia magna) | | LC50 fish 2 | 13000 mg/l 96 h; Salmo gairdneri (Oncorhynchus mykiss) | | EC50 Daphnia 2 | 10800 mg/l (24 h; Daphnia magna) | | Threshold limit other aquatic organisms 1 | 65 mg/l (72 h; Protozoa) | | Threshold limit algae 1 | 1450 mg/l (192 h; Microcystis aeruginosa; Growth rate) | | Threshold limit algae 2 | 5000 mg/l (168 h; Scenedesmus quadricauda; Growth rate) |

12.1. Toxicity

| Methanol (67-56-1) | | | LC50 fish 1 | 15400 mg/l (96 h; Lepomis macrochirus; Lethal) | | EC50 Daphnia 1 | >10000 mg/l (48 h; Daphnia magna; Lethal) | | LC50 fish 2 | 10800 mg/l 96 h; Salmo gairdneri (Oncorhynchus mykiss) | | EC50 Daphnia 2 | 24500 mg/l (48 h; Daphnia magna; Locomotor effect) | | Threshold limit other aquatic organisms 1 | 6600 mg/l (16 h; Pseudomonas putida) | | Threshold limit algae 1 | 530 mg/l (192 h; Microcystis aeruginosa) | | Threshold limit algae 2 | 8000 mg/l (168 h; Scenedesmus quadricauda) |

12.1. Toxicity

| Isopropanol (67-63-0) | | | LC50 fish 1 | 4200 mg/l (96 h; Rasbora heteromorpha; Flow-through system) | | EC50 Daphnia 1 | >10000 mg/l (48 h; Daphnia magna) | | LC50 fish 2 | 9640 mg/l (96 h; Pimephales promelas; Lethal) | | EC50 Daphnia 2 | 13299 mg/l (48 h; Daphnia magna) | | Threshold limit algae 1 | >1000 mg/l (72 h; Scenedesmus subspicatus; Growth rate) | | Threshold limit algae 2 | 1800 mg/l (72 h; Algae; Cell numbers) |

12.2. Persistence and degradability

12.2. Persistence and degradability

Not established.

Not established.

12.2. Persistence and degradability

Readily biodegradable in water. Biodegradable in the soil. Highly mobile in soil. Biochemical oxygen demand (BOD) | 0.8 - 0.967 g O₂/g substance Chemical oxygen demand (COD) | 1.70 g O₂/g substance ThOD | 2.10 g O₂/g substance BOD (% of ThOD) | 0.43 % ThOD

Readily biodegradable in water and soil. Highly mobile in soil.

12.2. Persistence and degradability

Readily biodegradable in water. Biodegradable in the soil. Highly mobile in soil. Biochemical oxygen demand (BOD) | 0.6 - 1.12 g O₂/g substance Chemical oxygen demand (COD) | 1.42 g O₂/g substance ThOD | 1.5 g O₂/g substance BOD (% of ThOD) | 0.8 % ThOD

Methanol is readily biodegradable in water and soil, and is highly mobile in soil.

12.2. Persistence and degradability

Readily biodegradable in water. Biodegradable in the soil. Biodegradable in the soil under anaerobic conditions. No test data on mobility of the substance available. Biochemical oxygen demand (BOD) | 1.19 g O₂/g substance Chemical oxygen demand (COD) | 2.23 g O₂/g substance ThOD | 2.40 g O₂/g substance BOD (% of ThOD) | 0.49 % ThOD

12.2. Persistence and degradability

Not established.

Not established.

12.3. Bioaccumulative potential

Bioaccumulative potential | Not established.

Not established.

12.3. Bioaccumulative potential

| Ethanol (64-17-5) | | | BCF fish 1 | 1 (72 h; Cyprinus carpio) | | Log Pow | -0.31 (Experimental value) | | Bioaccumulative potential | Low potential for bioaccumulation (Log Kow < 4). |

Low potential for bioaccumulation (Log Kow < 4).

12.3. Bioaccumulative potential

| Methanol (67-56-1) | | | BCF fish 1 | < 10 (72 h; Leuciscus idus) | | BCF fish 2 | 1 (72 h; Cyprinus carpio; Blood) | | Log Pow | -0.77 (Experimental value; Other) | | Bioaccumulative potential | Low potential for bioaccumulation (BCF < 500). |

Low potential for bioaccumulation (BCF < 500).

12.3. Bioaccumulative potential

| Isopropanol (67-63-0) | | | Log Pow | 0.05 (Experimental value) | | Bioaccumulative potential | Low potential for bioaccumulation (Log Kow < 4). |

Low potential for bioaccumulation (Log Kow < 4).

12.3. Bioaccumulative potential

Not established.

12.4. Mobility in soil

| Ethanol (64-17-5) | | | --- | --- | | Surface tension | 0.022 N/m (20 °C) | | Methanol (67-56-1) | | | Surface tension | 0.023 N/m (20 °C) | | Isopropanol (67-63-0) | | | Surface tension | 0.021 N/m (25 °C) |

12.5. Other adverse effects

Effect on the global warming : No known ecological damage caused by this product. Other information : Avoid release to the environment.

No known ecological damage caused by this product.

13

Disposal Considerations

Waste treatment methods, disposal recommendations, and waste codes

Disposal

Dispose in a safe manner in accordance with local/national regulations. Dispose of contents/container to comply with local, state and federal regulations.

14

Transport Information

UN numbers, shipping names, transport classes, and regulatory requirements

UN Number
UN1170
Shipping Name
Ethanol solutions
Transport Class
3
Packing Group
II
15

Regulatory Information

Chemical regulations, safety assessments, and compliance status

?

Frequently Asked Questions

Common questions about safety, handling, and properties

What is the hazard signal word for Safranin, 1% Alcoholic?

The signal word is danger.

What is the physical form of Safranin, 1% Alcoholic?

Safranin, 1% Alcoholic is a liquid with red color. It has alcohol odour odor.

What is the solubility of Safranin, 1% Alcoholic?

Soluble in water. Soluble in methanol. Soluble in isopropanol. Soluble in ethanol. Water: Solubility in water of component(s) of the mixture : • Ethanol: • Methanol: >= 100 g/100ml • Isopropanol:

What is Safranin, 1% Alcoholic used for?

For laboratory and manufacturing use only.

What are the hazard statements for Safranin, 1% Alcoholic?

This substance has 5 hazard statements:

  • H225: Highly flammable liquid and vapor
  • H319: Causes serious eye irritation
  • H350: May cause cancer
  • H361: Suspected of damaging fertility or the unborn child
  • H370: Causes damage to organs (central nervous system, optic nerve, liver, kidneys)

What is the melting point of Safranin, 1% Alcoholic?

The melting point is -115 °C and the boiling point is 78 °C.