2-ethylhexan-1-ol

12.1.1. Aquatic toxicity 12.1.1.1. SHORT-TERM TOXICITY LC50/96h Fish: Leuciscus idus melanotus; 17.1 mg/l LC50/96h Fish: Pimephales promelas; 28.2 mg/l EC50/48h Crustacea: Daphnia magna 39 mg/l EC50/72h A Algae: Scenedesmus subspicatus. 11.5 mg/l; biomass 16.6 mg/l; freshwater EC120/LC10 or NOEC (freshwater) 5.3 mg/l 12.1.1.2. LONG TERM TOXICITY According to REACH Annex IX, 9.1, Column 2, the test is not required (CSR does not indicate the need for further investigations) 12.1.2. Sediment toxicity The substance is readily biodegradable, the adsorption potential is low (Log Koc = 1.42), as is the bioconcentration factor (the estimated Log BCF was 1.4). Direct and indirect exposure to soil is not likely, since the substance is not intentionally applied to soil. Therefore, no testing is required in accordance with REACH; ANNEX X; No. 9.5.1, column 2. 12.1.3. Terrestrial toxicity 12.1.3.1. TOXICITY TO SOIL MICRO-ORGANISMS. The substance is readily biodegradable, and the adsorption potential is low (low Pow and Koc). Direct and indirect exposure to soil is not likely, since the substance is not intentionally applied to soil. Therefore, no testing is required in accordance with REACH; ANNEX X; No. 9.4, column 2. 12.1.3.2. TOXICITY TO SOIL MACRO-ORGANISMS Arthropods The substance is readily biodegradable, and the adsorption potential is low (low Pow and Koc). Direct and indirect exposure to soil is not likely, since the substance is not intentionally applied to soil. Therefore, no testing is required in accordance with REACH; ANNEX VIII; No. 9.4, column 2. Other The substance is readily biodegradable, and the adsorption potential is low (low Pow and Koc). Direct and indirect exposure to soil is not likely, since the substance is not intentionally applied to soil. Therefore, no testing is required in accordance with REACH; ANNEX X; No. 9.4, column 2. 12.1.3.3. TOXICITY TO TERRESTRIAL PLANTS The substance is readily biodegradable, and the adsorption potential is low (low Pow and Koc). Direct and indirect exposure to soil is not likely, since the substance is not intentionally applied to soil. Therefore, no testing is required in accordance with REACH; ANNEX VIII; No. 9.4, column 2. 12.1.3.4. TOXICITY TO TERRESTRIAL ANIMALS Birds: The substance is readily biodegradable, the adsorption potential is low (low Pow and Koc), as is the bioconcentration factor (the estimated Log BCF was 1.4). Direct and indirect exposure to soil is not likely, since the substance is not intentionally applied to soil. Therefore, no testing is required in accordance with REACH; ANNEX X; No. 9.6.1, column 2. Mammals: Toxicity in repeated dose toxicity studies with rodents was moderate. The NOAEL observed in a chronic study was 200 mg/kg bw/d. 12.1.4. Microbiological activity in sewage treatment systems The substance is readily biodegradable. Therefore, no testing is required in accordance with REACH; ANNEX VIII; No. 9.1.4, column 2. NOEC/24h (activated sludge, domestic) >300 mg/l

The substance is readily biodegradable.

The bioconcentration factor (the estimated Log BCF was 1.4).

The adsorption potential is low (Log Koc = 1.42).

## 12.2.1. Stability In accordance with Annex VIII, No. 9.2.2.1, column 2, no testing is required because the substance is readily biodegradable ### 12.2.1.1. HYDROLYSIS: 2-EH is not expected to hydrolyze in water due to the absence of hydrolysable groups. Since alcohols are generally resistant to hydrolysis and the substance structure does not contain other hydrolyzable elements, 2-ethylhexanol will not hydrolyze in water under environmental conditions (GUBDH, 2008). ## 12.2.2. Biodegradation ### 12.2.2.1. BIODEGRADATION IN WATER Biodegradation in water: readily biodegradable 2-ethylhexanol was readily biodegradable in a MITI-I Test, (equivalent to OECD TG 301-C). This is in line with the observation that the chemical oxygen demand (COD) was reduced by >95% in the Zahn-Wellens test (OECD 302B guideline; reliability 2) within 5 days demonstrating rapid biodegradation. Kinetic of test substance (in %; Hoechst, 1979): | Day | 1 | 2 | 5 | 7 | 9 | | --- | --- | --- | --- | --- | --- | | % degraded | 23 | 36 | 96 | 97 | 96 | According to ECHA Guidance on information requirements and chemical safety assessment, Chapter R.16.4.4. the degradation rate constant is approx. k = 4.7 x 10^-2 (d-1), with a half-life of 15 days. ### 12.2.2.2. BIODEGRADATION IN SOIL The substance is readily biodegradable. Therefore, no testing is required in accordance with REACH; ANNEX IX; No. 9.2.1.3, column 2.

Log Kow 2.9 BCF 25.33 Using an accepted calculation method (BCF program, version 2.17) the estimated Log BCF was 1.404 (BCF = 25.33) (GUBDH, 2008). In accordance with column 2 of Annex IX, due to the low log Pow of 2.9 for 2-Ethylhexanol (Perstorp, 2010) no bioaccumulation study is required. Potential for bioaccumulation is expected to be low. A BCF of 25.3 calculated by QSAR also points towards low bioaccumulation potential. In addition the substance is readily biodegradable (NITE, 2002). Therefore and for reasons of animal welfare a fish study is not performed.

### 12.4.1. Adsorption/desorption Using an accepted calculation program (PCKOC version 1.66, connectivity index method) the estimated Koc was 26, i.e. Log Koc=1.42 (GUBDH, 2008). In accordance with column 2 of Annex IX, the study does not need to be conducted if the substance has a low potential for bioaccumulation. The log Pow of 2.9 for 2-Ethylhexanol (Perstorp, 2010) indicates a low potential for bioaccumulation. Low values for Koc calculated with a QSAR tool also point to low absorption to soil. In addition the substance is readily biodegradable (NITE, 2002). ### 12.4.2. Volatilisation No information available ### 12.4.3. Distribution modelling No information available

Based on the available data it is concluded that 2-ethylhexanol - is readily biodegradable and does not fulfill the P or vP criterion - is not bioaccumulative and does not fulfill the B or vB criterion - does not fulfill the T criterion and therefore is evaluated to be not a PBT or vPvB substance.

No data.