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Safety Data Sheet EN

Gram's crystal violet solution for the Gram staining method

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01

Identification

Product identifiers, regulatory numbers, and supplier information

Product Information

Product Name
Gram's crystal violet solution for the Gram staining method
Product Code
1.09218

Regulatory Identifiers

CAS Number
N/A
REACH No.
This product is a mixture. REACH Registration Number see section 3.

Identified Uses

In vitro diagnostic reagent, Reagent for analysis

Manufacturers & Suppliers

M

Merck KGaA

manufacturer

Frankfurter Str. 250, D-64271 DARMSTADT

+49 (0)6151 72-0

Emergency Contacts

CHEMTREC (GB)

+(44)-870-8200418

CHEMTREC Ireland

+(353)-19014670

CHEMTREC India

001-803-017-9114

02

Hazard Identification

GHS classification, signal word, pictograms, and hazard statements

Classified warning

Hazard Classifications

Flam. Liq. Cat. 3 H226
Aquatic Chronic Cat. 3 H412

GHS Pictograms

GHS02 - Flammable

GHS02

Hazard Statements

H226 Flammable liquid and vapor.
H412 Harmful to aquatic life with long lasting effects.

Precautionary Statements

P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233 Keep container tightly closed.
P240 Ground and bond container and receiving equipment.
P241 Use explosion-proof electrical/ ventilating/ lighting/ equipment.
P242 Use non-sparking tools.
P273 Avoid release to the environment.

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03

Composition / Information on Ingredients

Chemical components, concentration ranges, and hazardous substance identification

Type mixture
Chemical Name CAS Number Concentration Hazardous
ethanol EC: 200-578-6 64-17-5 50% Yes
Crystal violet EC: 208-953-6 548-62-9 0.25 - 1% Yes
Phenol EC: 203-632-7 108-95-2 0.25 - 1% Yes
04

First Aid Measures

Emergency procedures for chemical exposure incidents

Inhalation

After inhalation: fresh air.

Skin contact

In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with water/ shower.

Eye contact

After eye contact: rinse out with plenty of water. Remove contact lenses.

Ingestion

After swallowing: make victim drink water (two glasses at most). Consult doctor if feeling unwell.

05

Firefighting Measures

Extinguishing media, specific hazards, and firefighter protection

Suitable media

Water, Foam, Carbon dioxide (CO2), Dry powder

Unsuitable media

For this substance/mixture no limitations of extinguishing agents are given.

Specific hazards

Carbon oxides. Mixture with combustible ingredients. Vapors are heavier than air and may spread along floors. Forms explosive mixtures with air at elevated temperatures. Development of hazardous combustion gases or vapours possible in the event of fire.

Instructions

Remove container from danger zone and cool with water.

Firefighter Protection

In the event of fire, wear self-contained breathing apparatus.

06

Accidental Release Measures

Spill cleanup procedures, containment, and environmental protection

Emergency procedures

Do not breathe vapors, aerosols. Ensure adequate ventilation. Keep away from heat and sources of ignition. Evacuate the danger area, observe emergency procedures, consult an expert.

Environmental

Do not let product enter drains. Risk of explosion.

Cleanup methods

Take up with liquid-absorbent material (e.g. Chemizorb®).

Materials: liquid-absorbent material (e.g. Chemizorb®)

Related Products

Similar products with comparable safety profiles

07

Handling and Storage

Safe handling precautions, storage conditions, and workplace requirements

Handling

Keep away from open flames, hot surfaces and sources of ignition. Take precautionary measures against static discharge.

Storage

Keep container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition. Recommended storage temperature see product label.

Hygiene

Change contaminated clothing. Wash hands after working with substance.

Fire prevention

Keep away from open flames, hot surfaces and sources of ignition. Take precautionary measures against static discharge.

08

Exposure Controls / PPE

Occupational exposure limits, engineering controls, and protective equipment

Hands

Full contact: Material: butyl-rubber, Minimum layer thickness: 0,7 mm, Break through time: 480 min, Material tested: Butoject® (KCL 898). Splash contact: Material: Nitrile rubber, Minimum layer thickness: 0,40 mm, Break through time: 120 min, Material tested: Camatril® (KCL 730 / Aldrich Z677442, Size M). Standards compliance: EN374.

Eyes

Safety glasses. Standards compliance: NIOSH (US) or EN 166(EU).

Respiratory

Recommended Filter type: ABEK. Required when vapours/aerosols are generated.

Skin/Body

Flame retardant antistatic protective clothing.

Environmental

Do not let product enter drains. Risk of explosion.

09

Physical and Chemical Properties

Appearance, physical state, melting point, boiling point, and material characteristics

Bulk Density
---
Evaporation Rate
---
Boiling Point
No data available
Physical State
liquid
Colour Intensity
---
Cloud Point
---
Form
---
Solubility In Fat
---
Upper Explosive Limit
No data available
Freezing Point
No data available
Density
0,99 g/cm³ at 20 °C
Properties Status
---
Partition Coefficient
No data available
Crystallisation Point
---
Solidification Point
---
Flash Point
47 °C
Odor Threshold
---
Explosive Limits
---
Odor
phenol-like
Solubility In Water
at 20 °C soluble
Voc Content
---
Decomposition Temperature
No data available
Physical State Data
liquid
Softening Point
---
Vapor Pressure
No data available
Molecular Weight
---
Flammability
No data available
Lower Explosive Limit
No data available
Metal Corrosion
---
State Under Standard Conditions
---
Dynamic Viscosity
No data available
Melting Point
No data available
Relative Evaporation Rate
---
Kinematic Viscosity
No data available
Solubility
---
Vapor Density
---
Auto Ignition Temperature
No data available
Colour
blue
Appearance
---
Relative Density
No data available
Ph
No data available
10

Stability and Reactivity

Chemical stability, hazardous reactions, and incompatible materials

Stability

The product is chemically stable under standard ambient conditions (room temperature).

Reactivity

Vapor/air-mixtures are explosive at intense warming.

Hazardous reactions

Violent reactions possible with: The generally known reaction partners of water.

Avoid

Heating.

Incompatible

No data available

Decomposition

In the event of fire: see section 5

12

Ecological Information

Environmental toxicity, biodegradation, and bioaccumulation data

The data for this section has not been processed yet.

13

Disposal Considerations

Waste treatment methods, disposal recommendations, and waste codes

Treatment

See www.retrologistik.com for processes regarding the return of chemicals and containers, or contact us there if you have further questions.

Disposal

See www.retrologistik.com for processes regarding the return of chemicals and containers, or contact us there if you have further questions.

14

Transport Information

UN numbers, shipping names, transport classes, and regulatory requirements

Shipping Name
Not dangerous goods
15

Regulatory Information

Chemical regulations, safety assessments, and compliance status

Chemical Safety Assessment Not performed
?

Frequently Asked Questions

Common questions about safety, handling, and properties

What is the hazard signal word for Gram's crystal violet solution for the Gram staining method?

The signal word is warning.

What is the physical form of Gram's crystal violet solution for the Gram staining method?

Gram's crystal violet solution for the Gram staining method is a liquid with blue color. It has phenol-like odor.

What is Gram's crystal violet solution for the Gram staining method used for?

In vitro diagnostic reagent, Reagent for analysis

What are the hazard statements for Gram's crystal violet solution for the Gram staining method?

This substance has 2 hazard statements:

  • H226: Flammable liquid and vapor.
  • H412: Harmful to aquatic life with long lasting effects.

What is the melting point of Gram's crystal violet solution for the Gram staining method?

The melting point is No data available and the boiling point is No data available.