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Safety Data Sheet EN

Aerospray® Gram Reagent C, Crystal Violet

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01

Identification

Product identifiers, regulatory numbers, and supplier information

Product Information

Product Name
Aerospray® Gram Reagent C, Crystal Violet
Product Form
Mixture
Product Code
SS-041C, SS-041C-EU, SS-141C

Regulatory Identifiers

CAS Number
N/A

Identified Uses

Gram staining Laboratory chemical

Uses advised against

No additional information available

Manufacturers & Suppliers

ELITechGroup Inc. logo

ELITechGroup Inc.

manufacturer

370 West 1700 South, US– 84321 Logan, UT – Cache, USA

+1 (435) 752-6011

http://www.elitechgroup.com

Emergency Contacts

InfoTrac Emergency Response

1-800-535-5053

InfoTrac Emergency Response

+1 352-323-3500

02

Hazard Identification

GHS classification, signal word, pictograms, and hazard statements

Classified no signal word

GHS Pictograms

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03

Composition / Information on Ingredients

Chemical components, concentration ranges, and hazardous substance identification

Type mixture
Chemical Name CAS Number Concentration Hazardous
C.I. basic violet 3 substance listed as REACH Candidate ([4-[4,4'-bis(dimethylamino) benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride (C.I. Basic Violet 3)) EC: 208-953-6 548-62-9 1% Yes
formaldehyde substance with a Community workplace exposure limit EC: 200-001-8 50-00-0 0.1% Yes
04

First Aid Measures

Emergency procedures for chemical exposure incidents

Inhalation

Remove person to fresh air and keep comfortable for breathing. Allow affected person to breathe fresh air. Allow the victim to rest.

Skin contact

Remove affected clothing and wash all exposed skin area with mild soap and water, followed by warm water rinse. Wash skin with plenty of water.

Eye contact

Rinse immediately with plenty of water. Obtain medical attention if pain, blinking or redness persists. Rinse eyes with water as a precaution.

Ingestion

Rinse mouth. Do NOT induce vomiting. Obtain emergency medical attention. Call a poison center or a doctor if you feel unwell.

Medical Treatment

Treat symptomatically.

05

Firefighting Measures

Extinguishing media, specific hazards, and firefighter protection

Suitable media

Foam. Dry powder. Carbon dioxide. Water spray. Sand.

Unsuitable media

Do not use a heavy water stream.

Instructions

Use water spray or fog for cooling exposed containers. Exercise caution when fighting any chemical fire. Prevent fire fighting water from entering the environment.

Firefighter Protection

Do not enter fire area without proper protective equipment, including respiratory protection. Do not attempt to take action without suitable protective equipment. Self-contained breathing apparatus. Complete protective clothing.

06

Accidental Release Measures

Spill cleanup procedures, containment, and environmental protection

Emergency procedures

Ventilate spillage area. Evacuate unnecessary personnel.

Environmental

Avoid release to the environment.

Cleanup methods

Take up liquid spill into absorbent material. Soak up spills with inert solids, such as clay or diatomaceous earth as soon as possible. Collect spillage. Store away from other materials.

Materials: inert solids, such as clay or diatomaceous earth

—

Related Products

Similar products with comparable safety profiles

A

Aerospray® Gram Reagent A, Decolorizer with Acetone and Fuchsin

---

C

Crystal Violet Grams Stain

---

G

Gram's crystal violet solution for the Gram staining method

---

A

Aersopray® Gram Reagent B, Iodine

---

A

Aerospray® TB Reagent C, Auramine O

---

A

Aerospray® TB Reagent A, Carbol Fuchsin Decolorizer

---

C

Crystal violet (C.I. 42555) indicator ACS, Reag. Ph Eur

CAS: 548-62-9

C

Crystal violet (C.I. 42555) indicator ACS,Reag. Ph Eur

CAS: 548-62-9

07

Handling and Storage

Safe handling precautions, storage conditions, and workplace requirements

Handling

Ensure good ventilation of the work station. Wear personal protective equipment. Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work. Provide good ventilation in process area to prevent formation of vapour.

Storage

Keep away from ignition sources. Protect from sunlight. Store in a well-ventilated place. Keep cool.

Hygiene

Do not eat, drink or smoke when using this product. Always wash hands after handling the product. Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.

Fire prevention

Keep away from ignition sources.

08

Exposure Controls / PPE

Occupational exposure limits, engineering controls, and protective equipment

Engineering

Ensure good ventilation of the work station.

Hands

Wear protective gloves. Suitable gloves should be tested to EN 374. The glove material has to be impermeable and resistant to the product/the substance/the preparation. As the product is a preparation of several substances, the resistance and penetration time/breakthrough time of the glove material cannot be calculated/observed in advance and, therefore, has to be checked prior to the application. The following are recommended: materials - natural latex or nitrile; thickness - 4 to 6 mils (0.1 mm - 0.15 mm); minimum breakthrough time - 60 minutes.

Eyes

Chemical goggles or safety glasses. Safety glasses

Respiratory

Wear appropriate mask

Skin/Body

Wear suitable protective clothing

Environmental

Avoid release to the environment.

09

Physical and Chemical Properties

Appearance, physical state, melting point, boiling point, and material characteristics

Language Code
---
Physical State
Liquid
Physical State Data
liquid
State Under Standard Conditions
---
Appearance
---
Colour
Purple
Colour Intensity
---
Form
---
Odor
Odourless
Odor Threshold
Not available
Ph
≈ 4.3 (4 – 4.5)
Melting Point
Not applicable
Boiling Point
Not available
Flash Point
> 93.3 °C
Freezing Point
Not available
Softening Point
---
Solidification Point
---
Cloud Point
---
Crystallisation Point
---
Relative Evaporation Rate
---
Evaporation Rate
---
Vapor Pressure
Not available
Vapor Density
---
Relative Density
Not available
Bulk Density
---
Density
0.9958 g/cm³
Flammability
Non flammable.
Upper Explosive Limit
Not available
Lower Explosive Limit
Not available
Explosive Limits
---
Auto Ignition Temperature
Not available
Decomposition Temperature
Not available
Solubility
Water: No data available
Partition Coefficient
Not available
Solubility In Water
---
Solubility In Fat
---
Molecular Weight
---
Voc Content
---
Metal Corrosion
---
Dynamic Viscosity
---
Kinematic Viscosity
Not available
Properties Status
---
10

Stability and Reactivity

Chemical stability, hazardous reactions, and incompatible materials

Stability

Not established.

Reactivity

The product is non-reactive under normal conditions of use, storage and transport.

Hazardous reactions

Not established.

Avoid

Direct sunlight. Extremely high or low temperatures.

Incompatible

Strong acids. Strong bases.

Decomposition

fume. Carbon monoxide. Carbon dioxide.

12

Ecological Information

Environmental toxicity, biodegradation, and bioaccumulation data

12.7. Other adverse effects

Additional information : Avoid release to the environment.

12.1. Toxicity

Ecology - general : The product is not considered harmful to aquatic organisms nor to cause long-term adverse effects in the environment. Hazardous to the aquatic environment, short-term (acute) : Not classified Hazardous to the aquatic environment, long-term (chronic) : Not classified C.I. basic violet 3 (548-62-9)

12.1. Toxicity

| LC50 - Fish [1] | 0.082 mg/l (96 h, Pimephales promelas, Static system, Fresh water, Experimental value, GLP) | | --- | --- | | EC50 - Crustacea [1] | 0.24 – 0.5 mg/l Test organisms (species): Daphnia magna | | EC50 72h - Algae [1] | 0.025 – 0.8 mg/l Test organisms (species): Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) | | ErC50 algae | 0.025 – 0.8 mg/l (OECD 201: Alga, Growth Inhibition Test, 72 h, Pseudokirchneriella subcapitata, Static system, Fresh water, Experimental value, Estimated value) |

12.1. Toxicity

| LC50 - Fish [1] | 6.7 mg/l (96 h, Morone saxatilis, Static system, Salt water, Experimental value, Lethal) | | --- | --- | | LC50 - Fish [2] | 62 (62 – 109) mg/l 96 h; Salmo gairdneri (Oncorhynchus mykiss) | | EC50 - Crustacea [1] | 5.8 mg/l (OECD 202: Daphnia sp. Acute Immobilisation Test, 48 h, Daphnia pulex, Static system, Fresh water, Experimental value, Locomotor effect) | | EC50 72h - Algae [1] | 3.48 mg/l Test organisms (species): Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) | | EC50 72h - Algae [2] | 4.89 mg/l Test organisms (species): Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) | | ErC50 algae | 4.89 – 6.61 mg/l (OECD 201: Alga, Growth Inhibition Test, 72 h, Desmodesmus subspicatus, Static system, Fresh water, Experimental value, Nominal concentration) | | NOEC chronic fish | ≥ 48 mg/l Test organisms (species): Oryzias latipes Duration: '28 d' |

12.2. Persistence and degradability

Aerospray® Gram Reagent C, Crystal Violet | Persistence and degradability | Not established.

Not established.

12.2. Persistence and degradability

| C.I. basic violet 3 (548-62-9) | | | Persistence and degradability | Not readily biodegradable in water. |

Not readily biodegradable in water.

12.2. Persistence and degradability

Readily biodegradable in water. Biochemical oxygen demand (BOD) 0.64 g O₂/g substance Chemical oxygen demand (COD) 1.06 g O₂/g substance ThOD 1.068 g O₂/g substance

12.3. Bioaccumulative potential

| Aerospray® Gram Reagent C, Crystal Violet | | | --- | --- | | Bioaccumulative potential | Not established. |

Bioaccumulative potential is not established for Aerospray® Gram Reagent C, Crystal Violet.

12.3. Bioaccumulative potential

| C.I. basic violet 3 (548-62-9) | | | BCF - Fish [1] | 3.8 – 16 l/kg (6 week(s), Cyprinus carpio, Static system, Fresh water, Experimental value) | | Partition coefficient n-octanol/water (Log Pow) | 1.172 (Experimental value, OECD 107: Partition Coefficient (n-octanol/water): Shake Flask Method, 25 °C) | | Bioaccumulative potential | Low potential for bioaccumulation (BCF < 500).

Low potential for bioaccumulation (BCF < 500).

12.3. Bioaccumulative potential

| formaldehyde (50-00-0) | | | BCF - Fish [1] | < 1 (1 h, Flow-through system, Salt water, Weight of evidence) | | Partition coefficient n-octanol/water (Log Pow) | 0.35 (Calculated, KOWWIN, 25 °C) | | Bioaccumulative potential | Low potential for bioaccumulation (BCF < 500). |

Low potential for bioaccumulation (BCF < 500).

12.4. Mobility in soil

12.4. Mobility in soil | C.I. basic violet 3 (548-62-9) | | | --- | --- | Surface tension | 44.2 mN/m (1 %) | Organic Carbon Normalized Adsorption Coefficient (Log Koc) | 5.79 (log Koc, Experimental value) | Ecology - soil | Adsorbs into the soil. | formaldehyde (50-00-0) | | | Surface tension | 73 mN/m (20 °C, Aqueous solution, 7.5 g/l) | Ecology - soil | Not applicable (gas). Toxic to flora.

12.5. Results of PBT and vPvB assessment

Component C.I. basic violet 3 (548-62-9) This substance/mixture does not meet the PBT criteria of REACH regulation, annex XIII This substance/mixture does not meet the vPvB criteria of REACH regulation, annex XIII formaldehyde (50-00-0) This substance/mixture does not meet the PBT criteria of REACH regulation, annex XIII This substance/mixture does not meet the vPvB criteria of REACH regulation, annex XIII

12.6. Endocrine disrupting properties

No additional information available
13

Disposal Considerations

Waste treatment methods, disposal recommendations, and waste codes

Treatment

Dispose of contents/container in accordance with licensed collector's sorting instructions.

Disposal

Dispose in a safe manner in accordance with local/national regulations.

14

Transport Information

UN numbers, shipping names, transport classes, and regulatory requirements

15

Regulatory Information

Chemical regulations, safety assessments, and compliance status

Regulatory information for Aerospray® Gram Reagent C, Crystal Violet

Chemical Safety Assessment Not performed
?

Frequently Asked Questions

Common questions about safety, handling, and properties

What is the hazard signal word for Aerospray® Gram Reagent C, Crystal Violet?

The signal word is no signal word.

What is the physical form of Aerospray® Gram Reagent C, Crystal Violet?

Aerospray® Gram Reagent C, Crystal Violet is a liquid with purple color. It has odourless odor.

What is the solubility of Aerospray® Gram Reagent C, Crystal Violet?

Water: No data available

What is Aerospray® Gram Reagent C, Crystal Violet used for?

Gram staining Laboratory chemical

What is the melting point of Aerospray® Gram Reagent C, Crystal Violet?

The melting point is Not applicable and the boiling point is Not available.