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Safety Data Sheet EN

Papanicolaou's solution 3a polychromatic solution EA 31 for cytology

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01

Identification

Product identifiers, regulatory numbers, and supplier information

Product Information

Product Name
Papanicolaou's solution 3a polychromatic solution EA 31 for cytology
Product Code
1.09271, 109271

Regulatory Identifiers

CAS Number
N/A

Identified Uses

In vitro diagnostic reagent Reagent for analysis

Uses advised against

This product is not intended for consumer use.

Manufacturers & Suppliers

M

Merck Pty Ltd.

national supplier

259 Davidson Road, Corner Peddie Road WADEVILLE, GERMISTON 1428 SOUTH AFRICA

+27 (0) 8600 63725

Emergency Contacts

CHEMTREC

0-800-983-611

02

Hazard Identification

GHS classification, signal word, pictograms, and hazard statements

Classified danger

Hazard Classifications

Flam. Liq. Cat. 2 H225
Acute Tox. Cat. 4 H302
Acute Tox. Cat. 4 H332
Acute Tox. Cat. 4 H312
Eye Irrit. Cat. 2 H319
Skin Sens. Cat. 1 H317
STOT SE Cat. 1 H370

GHS Pictograms

GHS02 - Flammable

GHS02

GHS07 - Health hazard

GHS07

GHS08 - Serious health hazard

GHS08

Hazard Statements

H225 Highly flammable liquid and vapour.
H302 + H312 + H332 Harmful if swallowed, in contact with skin or if inhaled.
H317 May cause an allergic skin reaction.
H319 Causes serious eye irritation.
H370 Causes damage to organs (Eyes, Central nervous system).

Precautionary Statements

P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.
P301 + P312 IF SWALLOWED: Call a POISON CENTER/ doctor if you feel unwell.
P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P308 + P311 IF exposed or concerned: Call a POISON CENTER/ doctor.
IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/ doctor if you feel unwell.

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03

Composition / Information on Ingredients

Chemical components, concentration ranges, and hazardous substance identification

Type mixture
Chemical Name CAS Number Concentration Hazardous
ethanol EC: 200-578-6 64-17-5 70 - 90% Yes
Methanol EC: 200-659-6 67-56-1 10 - 20% Yes
ethylene glycol EC: 203-473-3 107-21-1 1 - 10% Yes
Eosin G EC: 241-409-6 17372-87-1 0.1 - 1% Yes
04

First Aid Measures

Emergency procedures for chemical exposure incidents

Inhalation

fresh air. Immediately call in physician. If breathing stops: immediately apply artificial respiration, if necessary also oxygen.

Skin contact

Take off immediately all contaminated clothing. Rinse skin with water/ shower. Call a physician immediately.

Eye contact

rinse out with plenty of water. Call in ophthalmologist. Remove contact lenses.

Ingestion

fresh air. Make victim drink ethanol (e.g. 1 drinking glass of a 40% alcoholic beverage). Call a doctor immediately (mention methanol ingestion). Only in exceptional cases, if no medical care is available within one hour, induce vomiting (only in fully conscious persons) and make victim drink ethanol again (approx. 0.3 ml of a 40% alcoholic beverage/kg body weight/hour). Consult a physician.

Immediate Medical Attention

Immediately call in physician. Call a physician immediately. Call a doctor immediately (mention methanol ingestion).

05

Firefighting Measures

Extinguishing media, specific hazards, and firefighter protection

Suitable media

Water, Foam, Carbon dioxide (CO2), Dry powder

Unsuitable media

For this substance/mixture no limitations of extinguishing agents are given.

Specific hazards

Combustible. Pay attention to flashback. Vapours are heavier than air and may spread along floors. Development of hazardous combustion gases or vapours possible in the event of fire. Forms explosive mixtures with air at ambient temperatures.

Instructions

Remove container from danger zone and cool with water.

Firefighter Protection

Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by keeping a safe distance or by wearing suitable protective clothing.

06

Accidental Release Measures

Spill cleanup procedures, containment, and environmental protection

Emergency procedures

Do not breathe vapours, aerosols. Avoid substance contact. Ensure adequate ventilation. Keep away from heat and sources of ignition. Evacuate the danger area, observe emergency procedures, consult an expert.

Environmental

Do not let product enter drains.

Cleanup methods

Take up carefully with liquid-absorbent material (e.g. Chemizorb®). Clean up affected area.

Materials: liquid-absorbent material (e.g. Chemizorb®)

—

Related Products

Similar products with comparable safety profiles

07

Handling and Storage

Safe handling precautions, storage conditions, and workplace requirements

Handling

Work under hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols.

Storage

Keep locked up or in an area accessible only to qualified or authorised persons. Keep container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition.

Hygiene

Immediately change contaminated clothing. Apply preventive skin protection. Wash hands and face after working with substance.

Fire prevention

Keep away from open flames, hot surfaces and sources of ignition.

08

Exposure Controls / PPE

Occupational exposure limits, engineering controls, and protective equipment

Hands

Material : butyl-rubber, Break through time : 480 min, Glove thickness : 0,7 mm, Protective index : Full contact, Manufacturer : Butoject® (KCL 898); Material : Viton®, Break through time : 120 min, Glove thickness : 0,70 mm, Protective index : Splash contact, Manufacturer : Vitoject® (KCL 890 / Aldrich Z677698, Size M). Remarks : This recommendation applies only to the product stated in the safety data sheet, supplied by us and for the designated use. When dissolving in or mixing with other substances and under conditions deviating from those stated in EN 16523-1 please contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell, Internet: www.kcl.de).

Eyes

Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Safety glasses

Respiratory

required when vapours/aerosols are generated. Our recommendations on filtering respiratory protection are based on the following standards: DIN EN 143, DIN 14387 and other accompanying standards relating to the used respiratory protection system. Recommended Filter : filter ABEK type: The entrepreneur has to ensure that maintenance, cleaning and testing of respiratory protective devices are carried out according to the instructions of the producer. These measures have to be properly documented. Filter type ABEK

Skin/Body

Flame retardant antistatic protective clothing.

Environmental

Do not let product enter drains. Risk of explosion.

09

Physical and Chemical Properties

Appearance, physical state, melting point, boiling point, and material characteristics

Language Code
---
Physical State
liquid
Physical State Data
liquid
State Under Standard Conditions
---
Appearance
---
Colour
greenish-blue
Colour Intensity
---
Form
---
Odor
of ethanol
Odor Threshold
---
Ph
5,0 - 5,5 (20 °C)
Melting Point
No data available
Boiling Point
No data available
Flash Point
17 °C
Freezing Point
---
Softening Point
---
Solidification Point
---
Cloud Point
---
Crystallisation Point
---
Relative Evaporation Rate
---
Evaporation Rate
No data available
Vapor Pressure
No data available
Vapor Density
---
Relative Density
No data available
Bulk Density
---
Density
0,82 g/cm3 (20 °C)
Flammability
---
Upper Explosive Limit
No data available
Lower Explosive Limit
No data available
Explosive Limits
---
Auto Ignition Temperature
No data available
Decomposition Temperature
No data available
Solubility
---
Partition Coefficient
---
Solubility In Water
(20 °C) soluble
Solubility In Fat
---
Molecular Weight
---
Voc Content
---
Metal Corrosion
---
Dynamic Viscosity
No data available
Kinematic Viscosity
No data available
Properties Status
---
10

Stability and Reactivity

Chemical stability, hazardous reactions, and incompatible materials

Stability

The product is chemically stable under standard ambient conditions (room temperature).

Reactivity

Vapours may form explosive mixture with air.

Hazardous reactions

Risk of explosion/exothermic reaction with: hydrogen peroxide, perchlorates, perchloric acid, Nitric acid, mercury(II) nitrate, permanganic acid, Nitriles, peroxi compounds, Strong oxidizing agents, nitrosyl compounds, Peroxides, sodium, Potassium, halogen oxides, calcium hypochlorite, nitrogen dioxide, metallic oxides, uranium hexafluoride, iodides, Chlorine, Alkali metals, Alkaline earth metals, alkali oxides, Ethylene oxide, salts of oxyhalogenic acids, nitrogen oxides, nonmetallic oxides, chromosulfuric acid, chlorates, hydrides, zinc diethyl, Halogens, powdered magnesium, Sulphuric acid, sodium hypochlorite, silver, silver compounds, Ammonia, potassium permanganate, conc. sulfuric acid. Risk of ignition or formation of inflammable gases or vapours with: halogen-halogen compounds, chromium(VI) oxide, chromyl chloride, Fluorine, hydrides, Oxides of phosphorus, platinum, Nitric acid with potassium permanganate.

Avoid

Warming.

Incompatible

hydrogen peroxide, perchlorates, perchloric acid, Nitric acid, mercury(II) nitrate, permanganic acid, Nitriles, peroxi compounds, Strong oxidizing agents, nitrosyl compounds, Peroxides, sodium, Potassium, halogen oxides, calcium hypochlorite, nitrogen dioxide, metallic oxides, uranium hexafluoride, iodides, Chlorine, Alkali metals, Alkaline earth metals, alkali oxides, Ethylene oxide, salts of oxyhalogenic acids, nitrogen oxides, nonmetallic oxides, chromosulfuric acid, chlorates, hydrides, zinc diethyl, Halogens, powdered magnesium, Sulphuric acid, sodium hypochlorite, silver, silver compounds, Ammonia, potassium permanganate, conc. sulfuric acid, halogen-halogen compounds, chromium(VI) oxide, chromyl chloride, Fluorine, Oxides of phosphorus, platinum, Nitric acid with potassium permanganate.

Decomposition

In the event of fire: see section 5

12

Ecological Information

Environmental toxicity, biodegradation, and bioaccumulation data

12.1 Toxicity

Components: ethanol: Toxicity to fish : LC50 (Pimephales promelas (fathead minnow)): 15.300 mg/l End point: mortality Exposure time: 96 h Test Type: flow-through test Analytical monitoring: yes Method: US-EPA Toxicity to daphnia and other aquatic invertebrates : LC50 (Ceriodaphnia dubia (water flea)): 5.012 mg/l End point: mortality Exposure time: 48 h Test Type: static test Remarks: (ECHA) Toxicity to algae/aquatic plants : ErC50 (Chlorella vulgaris (Fresh water algae)): 275 mg/l Exposure time: 72 h Test Type: static test Method: OECD Test Guideline 201 Toxicity to microorganisms : IC50 (activated sludge): > 1.000 mg/l Exposure time: 3 h Test Type: static test Analytical monitoring: yes Method: OECD Test Guideline 209 The value is given in analogy to the following substances: Methanol Toxicity to fish (Chronic toxicity) : NOEC: 250 mg/l Exposure time: 120 h Species: Danio rerio (zebra fish) Test Type: semi-static test Remarks: (ECHA) Toxicity to daphnia and other aquatic invertebrates (Chronic toxicity) : NOEC: 9,6 mg/l End point: reproduction rate Exposure time: 9 d Species: Daphnia magna (Water flea) Test Type: semi-static test Remarks: (ECHA)

12.1 Toxicity

## Methanol: Toxicity to fish : LC50 (Lepomis macrochirus (Bluegill)): 15.400,0 mg/l End point: mortality Exposure time: 96 h Test Type: flow-through test Analytical monitoring: yes Method: US-EPA Toxicity to daphnia and other aquatic invertebrates : EC50 (Daphnia magna (Water flea)): 18.260 mg/l End point: Immobilization Exposure time: 96 h Test Type: semi-static test Method: OECD Test Guideline 202 Toxicity to algae/aquatic plants : ErC50 (Pseudokirchneriella subcapitata (green algae)): ca. 22.000,0 mg/l Exposure time: 96 h Test Type: static test Method: OECD Test Guideline 201 Toxicity to microorganisms : IC50 (activated sludge): > 1.000 mg/l Exposure time: 3 h Test Type: static test Analytical monitoring: yes Method: OECD Test Guideline 209 Toxicity to fish (Chronic toxicity) : NOEC: 7.900 mg/l Exposure time: 200 h Species: Oryzias latipes (Orange-red killifish) Remarks: (External MSDS)

12.1 Toxicity

12.1 Toxicity ## ethylene glycol: Toxicity to fish : LC50 (Pimephales promelas (fathead minnow)): 72.860 mg/l End point: mortality Exposure time: 96 h Test Type: static test Method: US-EPA Millipore- 1.09271 The life science business of Merck operates as MilliporeSigma in the US and Canada Page 18 of 27 Toxicity to daphnia and other aquatic invertebrates : EC50 (Daphnia magna (Water flea)): > 100 mg/l Exposure time: 48 h Test Type: static test Analytical monitoring: yes Method: OECD Test Guideline 202 GLP: yes Toxicity to algae/aquatic plants : NOEC (Pseudokirchneriella subcapitata (green algae)): > 100 mg/l Exposure time: 72 h Test Type: static test Analytical monitoring: yes Method: OECD Test Guideline 201 GLP: yes Toxicity to fish (Chronic toxicity) : LC50: > 1.500 mg/l End point: mortality Exposure time: 28 d Species: Menidia peninsulae (tidewater silverside) Test Type: flow-through test Analytical monitoring: yes Remarks: (ECHA) The value is given in analogy to the following substances: The value is given in analogy to the following substances: triethylene glycol Toxicity to daphnia and other aquatic invertebrates (Chronic toxicity) : NOEC: 8.590 mg/l End point: reproduction rate Exposure time: 7 d Species: Ceriodaphnia dubia (water flea) Test Type: semi-static test Method: US-EPA

12.1 Toxicity

Eosin G: Toxicity to fish : LC50 (Oryzias latipes (Orange-red killifish)): 1.200 mg/l Exposure time: 48 h Remarks: (ECOTOX Database) Toxicity to daphnia and other aquatic invertebrates : EC50 (Daphnia magna (Water flea)): > 100 mg/l End point: Immobilization Exposure time: 48 h Test Type: static test Method: OECD Test Guideline 202 Toxicity to algae/aquatic plants : ErC50 (Desmodesmus subspicatus (green algae)): 51,3 mg/l Exposure time: 72 h Test Type: static test Method: OECD Test Guideline 201 Toxicity to : NOEC (Bacteria): 100 mg/l Millipore- 1.09271 The life science business of Merck operates as MilliporeSigma in the US and Canada Page 19 of 27 microorganisms End point: Growth rate Exposure time: 250 min Remarks: (ECHA)

12.2 Persistence and degradability

12.2 Persistence and degradability

Components: ethanol: Biodegradability : Test Type: aerobic Inoculum: activated sludge, non-adapted Result: Readily biodegradable. Biodegradation: ca. 95 % Exposure time: 15 d Method: OECD Test Guideline 301E Biochemical Oxygen Demand (BOD) : 930 - 1.670 mg/g Incubation time: 5 d Remarks: (Lit.) ThOD : 2.100 mg/g Remarks: (Lit.)

12.2 Persistence and degradability

### Methanol: **Biodegradability** : Result: Readily biodegradable. Biodegradation: 99 % Exposure time: 30 d Method: OECD Test Guideline 301D **Biochemical Oxygen Demand (BOD)** : 600 - 1.120 mg/g Incubation time: 5 d Remarks: (IUCLID) **Chemical Oxygen Demand (COD)** : 1.420 mg/g Remarks: (IUCLID) **ThOD** : 1.500 mg/g Remarks: (Lit.) **BOD/ThOD** : 76 % Remarks: Closed Bottle test (IUCLID) **Stability in water** : Hydrolysis: 83 - 91 % at 19 °C(72 h) Remarks: Hydrolyses on contact with water. Hydrolyses readily. Degradation half life: 2,2 yr Remarks: reaction with hydroxyl radicals (IUCLID) **Photodegradation** : Degradation (direct photolysis): 50 % Degradation half life: 17,2 d

12.2 Persistence and degradability

ethylene glycol: Biodegradability : Test Type: aerobic Inoculum: activated sludge, non-adapted Concentration: 53 mg/l Result: Readily biodegradable. Biodegradation: 90 - 100 % Exposure time: 10 d Method: OECD Test Guideline 301A GLP: yes Biochemical Oxygen Demand (BOD) : 780 mg/g Incubation time: 5 d Remarks: (IUCLID) Chemical Oxygen Demand (COD) : 1.190 mg/g Remarks: (IUCLID) ThOD : 1.290 mg/g Remarks: (IUCLID) BOD/ThOD : 60 % Remarks: (IUCLID)

12.2 Persistence and degradability

Eosin G: Biodegradability : Test Type: aerobic Concentration: 4 mg/l Result: Readily biodegradable. Biodegradation: 94,56 % Exposure time: 28 d Method: OECD Test Guideline 301D

12.3 Bioaccumulative potential

12.3 Bioaccumulative potential

Components: ethanol: Bioaccumulation : Remarks: Due to the distribution coefficient n-octanol/water, accumulation in organisms is not expected. Partition coefficient: n-octanol/water : log Pow: -0,35 (24 °C) pH: 7,4 Method: OECD Test Guideline 107 Remarks: Bioaccumulation is not expected.

12.3 Bioaccumulative potential

Methanol: Bioaccumulation : Species: Cyprinus carpio (Carp) Exposure time: 72 d Temperature: 20 °C Concentration: 5 mg/l Bioconcentration factor (BCF): 1,0 Millipore- 1.09271 Page 21 of 27 The life science business of Merck operates as MilliporeSigma in the US and Canada Partition coefficient: n-octanol/water : log Pow: -0,77 (25 °C) Method: (experimental) Remarks: (HSDB) Bioaccumulation is not expected.

Bioaccumulation is not expected for Methanol.

12.3 Bioaccumulative potential

**ethylene glycol:** Bioaccumulation : Remarks: Does not bioaccumulate. Partition coefficient: n-octanol/water : log Pow: -1,36 Remarks: Bioaccumulation is not expected.

12.3 Bioaccumulative potential

**Eosin G:** Partition coefficient: n-octanol/water : log Pow: -1,33 Remarks: Bioaccumulation is not expected.

12.4 Mobility in soil

Components:

12.4 Mobility in soil

Methanol: Stability in soil : Remarks: Will not adsorb on soil.

Methanol will not adsorb on soil.

12.5 Results of PBT and vPvB assessment

Assessment : This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher. **Components:**

The substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.

12.5 Results of PBT and vPvB assessment

ethanol: Assessment : Substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No 1907/2006, Annex XIII.

Substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No 1907/2006, Annex XIII.

12.5 Results of PBT and vPvB assessment

Methanol: Assessment : Not persistent, bioaccumulative, and toxic (PBT).

The substance is assessed as not persistent, bioaccumulative, and toxic (PBT).

12.5 Results of PBT and vPvB assessment

ethylene glycol: Assessment : Substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No 1907/2006, Annex XIII.

Substance does not meet the criteria for PBT or vPvB according to Regulation (EC) No 1907/2006, Annex XIII.

12.6 Endocrine disrupting properties

The substance/mixture does not contain components considered to have endocrine disrupting properties according to REACH Article 57(f) or Commission Delegated regulation (EU) 2017/2100 or Commission Regulation (EU) 2018/605 at levels of 0.1% or higher.

The substance/mixture does not contain components considered to have endocrine disrupting properties at levels of 0.1% or higher.

12.7 Other adverse effects

Product: Additional ecological information : Discharge into the environment must be avoided. Components:

Discharge into the environment must be avoided.

12.7 Other adverse effects

Methanol: Additional ecological information : Avoid release to the environment.

Avoid release to the environment.

13

Disposal Considerations

Waste treatment methods, disposal recommendations, and waste codes

Treatment

Waste material must be disposed of in accordance with the national and local regulations.

Disposal

Leave chemicals in original containers. No mixing with other waste.

14

Transport Information

UN numbers, shipping names, transport classes, and regulatory requirements

UN Number
1993
Shipping Name
FLAMMABLE LIQUID, N.O.S. (Methanol, ethanol)
Transport Class
3
Packing Group
II
15

Regulatory Information

Chemical regulations, safety assessments, and compliance status

?

Frequently Asked Questions

Common questions about safety, handling, and properties

What is the hazard signal word for Papanicolaou's solution 3a polychromatic solution EA 31 for cytology?

The signal word is danger.

What is the physical form of Papanicolaou's solution 3a polychromatic solution EA 31 for cytology?

Papanicolaou's solution 3a polychromatic solution EA 31 for cytology is a liquid with greenish-blue color. It has of ethanol odor.

What is Papanicolaou's solution 3a polychromatic solution EA 31 for cytology used for?

In vitro diagnostic reagent Reagent for analysis

What are the hazard statements for Papanicolaou's solution 3a polychromatic solution EA 31 for cytology?

This substance has 5 hazard statements:

  • H225: Highly flammable liquid and vapour.
  • H302 + H312 + H332: Harmful if swallowed, in contact with skin or if inhaled.
  • H317: May cause an allergic skin reaction.
  • H319: Causes serious eye irritation.
  • H370: Causes damage to organs (Eyes, Central nervous system).

What is the melting point of Papanicolaou's solution 3a polychromatic solution EA 31 for cytology?

The melting point is No data available and the boiling point is No data available.