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Safety Data Sheet EN

NBT

CAS 298-83-9
EC 206-067-4

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01

Identification

Product identifiers, regulatory numbers, and supplier information

Product Information

Product Name
NBT
Product Form
Powder.
Product Code
0034035 1896851
Chemical Name
2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2)
Synonyms
2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2); 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-, dichloride; Nitro blue tetrazolium; Nitrotetrazolium chloride blue; 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride; 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-,dichloride

Regulatory Identifiers

CAS Number
298-83-9 Check regulations →
EC Number
206-067-4 Check regulations →

Manufacturers & Suppliers

T

Thermo Fisher Scientific Europe

distributor

Perbio Science, Industriezone III, Industrielaan 27, 9320 Erembodegem Belgium

T

Thermo Fisher Scientific

manufacturer

Pierce Biotechnology, P.O. Box 117, Rockford, IL 61105, United States

815.968.0747

Emergency Contacts

CHEMTREC

703-527-3887

CHEMTREC UK

+(44) 870 8200418

National Poisons Information Service (UK Only)

0870 600 6266

02

Hazard Identification

GHS classification, signal word, pictograms, and hazard statements

Classified warning

Hazard Classifications

Acute Tox. Cat. 4 H302
Skin Irrit. Cat. 2 H315
Eye Irrit. Cat. 2 H319

GHS Pictograms

GHS07 - Health hazard

GHS07

Hazard Statements

H302 Harmful if swallowed.
H315 Causes skin irritation.
H319 Causes serious eye irritation.

Precautionary Statements

Wear protective gloves.
Wear eye or face protection.
Do not eat, drink or smoke when using this product.
IF SWALLOWED: Call a POISON CENTER or physician if you feel unwell.
IF IN EYES: Rinse cautiously with water for several minutes.
Dispose of contents and container in accordance with all local, regional, national and international regulations.

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03

Composition / Information on Ingredients

Chemical components, concentration ranges, and hazardous substance identification

Type substance

There are no additional ingredients present which, within the current knowledge of the supplier, are classified and contribute to the classification of the substance and hence require reporting in this section.

Chemical Name CAS Number Concentration Hazardous
5,5'-diphenyl-3,3'-bis (4-nitrophenyl)-2,2'-(3, 3'-dimethoxybiphenyl-4,4'-ylene) ditetrazolium dichloride EC: 206-067-4 298-83-9 98 - 100% Yes
04

First Aid Measures

Emergency procedures for chemical exposure incidents

Inhalation

Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband.

Symptoms: Exposure to airborne concentrations above statutory or recommended exposure limits may cause irritation of the nose, throat and lungs. Adverse symptoms may include the following: respiratory tract irritation, coughing.

Skin contact

Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention. Wash clothing before reuse. Clean shoes thoroughly before reuse.

Symptoms: Causes skin irritation. Adverse symptoms may include the following: irritation, redness.

Eye contact

Immediately flush eyes with plenty of water, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. Get medical attention.

Symptoms: Causes serious eye irritation. Adverse symptoms may include the following: pain or irritation, watering, redness.

Ingestion

Wash out mouth with water. Remove dentures if any. Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention. If necessary, call a poison center or physician. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband.

Symptoms: Harmful if swallowed. Irritating to mouth, throat and stomach.

Immediate Medical Attention

Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately.

Medical Treatment

In case of inhalation of decomposition products in a fire, symptoms may be delayed. The exposed person may need to be kept under medical surveillance for 48 hours.

05

Firefighting Measures

Extinguishing media, specific hazards, and firefighter protection

Suitable media

Use dry chemical powder.

Unsuitable media

Do not use water jet.

Specific hazards

Fine dust clouds may form explosive mixtures with air.

Instructions

Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Move containers from fire area if this can be done without risk. Use water spray to keep fire-exposed containers cool.

Firefighter Protection

Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. Clothing for fire-fighters (including helmets, protective boots and gloves) conforming to European standard EN 469 will provide a basic level of protection for chemical incidents.

06

Accidental Release Measures

Spill cleanup procedures, containment, and environmental protection

Emergency procedures

Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Shut off all ignition sources. No flares, smoking or flames in hazard area. Avoid breathing dust. Provide adequate ventilation.

Small spill

Move containers from spill area. Use spark-proof tools and explosion-proof equipment. Avoid dust generation. Using a vacuum with HEPA filter will reduce dust dispersal. Place spilled material in a designated, labeled waste container. Dispose of via a licensed waste disposal contractor.

Large spill

Move containers from spill area. Use spark-proof tools and explosion-proof equipment. Approach release from upwind. Prevent entry into sewers, water courses, basements or confined areas. Avoid dust generation. Do not dry sweep. Vacuum dust with equipment fitted with a HEPA filter and place in a closed, labeled waste container. Avoid creating dusty conditions and prevent wind dispersal. Dispose of via a licensed waste disposal contractor.

Environmental

Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers.

Cleanup methods

Using a vacuum with HEPA filter. Place spilled material in a designated, labeled waste container.

Related Products

Similar products with comparable safety profiles

07

Handling and Storage

Safe handling precautions, storage conditions, and workplace requirements

Handling

Put on appropriate personal protective equipment (see Section 8). Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing dust. Avoid the creation of dust when handling and avoid all possible sources of ignition (spark or flame). Prevent dust accumulation. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Electrical equipment and lighting should be protected to appropriate standards to prevent dust coming into contact with hot surfaces, sparks or other ignition sources. Take precautionary measures against electrostatic discharges. To avoid fire or explosion, dissipate static electricity during transfer by grounding and bonding containers and equipment before transferring material. Empty containers retain product residue and can be hazardous. Do not reuse container.

Storage

Store in a segregated and approved area. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials and food and drink. Eliminate all ignition sources. Separate from oxidizing materials. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers.

Hygiene

Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas.

Fire prevention

Avoid all possible sources of ignition (spark or flame). Electrical equipment and lighting should be protected to appropriate standards to prevent dust coming into contact with hot surfaces, sparks or other ignition sources. Take precautionary measures against electrostatic discharges. Dissipate static electricity during transfer by grounding and bonding containers and equipment.

08

Exposure Controls / PPE

Occupational exposure limits, engineering controls, and protective equipment

Engineering

Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. The engineering controls also need to keep gas, vapor or dust concentrations below any lower explosive limits. Use explosion-proof ventilation equipment.

Hands

Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Considering the parameters specified by the glove manufacturer, check during use that the gloves are still retaining their protective properties. It should be noted that the time to breakthrough for any glove material may be different for different glove manufacturers. In the case of mixtures, consisting of several substances, the protection time of the gloves cannot be accurately estimated.

Eyes

Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: chemical splash goggles. If operating conditions cause high dust concentrations to be produced, use dust goggles.

Respiratory

Use a properly fitted, particulate filter respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator.

Skin/Body

Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Appropriate footwear and any additional skin protection measures should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product.

Environmental

Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels.

09

Physical and Chemical Properties

Appearance, physical state, melting point, boiling point, and material characteristics

Odor Threshold
Not available.
Form
[Crystalline powder.]
Vapor Pressure
Not available.
Crystallisation Point
---
Decomposition Temperature
200°C
Cloud Point
---
State Under Standard Conditions
---
Dynamic Viscosity
---
Evaporation Rate
Not available.
Properties Status
---
Appearance
Solid. [Crystalline powder.]
Solubility In Fat
---
Solidification Point
---
Partition Coefficient
Not available.
Physical State
Solid. [Crystalline powder.]
Explosive Limits
---
Lower Explosive Limit
Not available.
Relative Density
Not available.
Bulk Density
---
Freezing Point
189°C
Flash Point
[Product does not sustain combustion.]
Softening Point
---
Solubility In Water
10 g/l
Vapor Density
28.1 [Air = 1]
Colour Intensity
Light
Relative Evaporation Rate
---
Melting Point
189°C
Boiling Point
---
Density
---
Kinematic Viscosity
---
Physical State Data
solid
Colour
Yellow
Upper Explosive Limit
Not available.
Ph
Not available.
Solubility
Partially soluble in the following materials: cold water, hot water and methanol.
Metal Corrosion
---
Auto Ignition Temperature
Not available.
Voc Content
---
Flammability
Not available.
Odor
Odorless.
Molecular Weight
---
10

Stability and Reactivity

Chemical stability, hazardous reactions, and incompatible materials

Stability

The product is stable.

Reactivity

No specific test data related to reactivity available for this product or its ingredients.

Hazardous reactions

Under normal conditions of storage and use, hazardous reactions will not occur.

Avoid

Avoid the creation of dust when handling and avoid all possible sources of ignition (spark or flame). Take precautionary measures against electrostatic discharges. To avoid fire or explosion, dissipate static electricity during transfer by grounding and bonding containers and equipment before transferring material. Prevent dust accumulation.

Incompatible

Reactive or incompatible with the following materials: oxidizing materials

Decomposition

Under normal conditions of storage and use, hazardous decomposition products should not be produced.

12

Ecological Information

Environmental toxicity, biodegradation, and bioaccumulation data

The data for this section has not been processed yet.

13

Disposal Considerations

Waste treatment methods, disposal recommendations, and waste codes

Treatment

The generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor.

Disposal

Dispose of surplus and non-recyclable products via a licensed waste disposal contractor.

14

Transport Information

UN numbers, shipping names, transport classes, and regulatory requirements

UN Number
UN2811
Shipping Name
Toxic solids, organic, n.o.s.
Transport Class
6.1
Packing Group
III
ADR

Transport within user's premises: always transport in closed containers that are upright and secure. Ensure that persons transporting the product know what to do in the event of an accident or spillage.

IMDG

Transport within user's premises: always transport in closed containers that are upright and secure. Ensure that persons transporting the product know what to do in the event of an accident or spillage.

IATA

Transport within user's premises: always transport in closed containers that are upright and secure. Ensure that persons transporting the product know what to do in the event of an accident or spillage.

15

Regulatory Information

Chemical regulations, safety assessments, and compliance status

Chemical Safety Assessment Not performed
?

Frequently Asked Questions

Common questions about safety, handling, and properties

What other names is NBT known by?

NBT is also known as: 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2); 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-, dichloride; Nitro blue tetrazolium; Nitrotetrazolium chloride blue; 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride; 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-,dichloride

What is the hazard signal word for NBT?

The signal word is warning.

What is the physical form of NBT?

NBT is a solid. [crystalline powder.] appearing as solid. [crystalline powder.] with yellow color. It has odorless. odor.

What is the solubility of NBT?

Partially soluble in the following materials: cold water, hot water and methanol.

What are the hazard statements for NBT?

This substance has 3 hazard statements:

  • H302: Harmful if swallowed.
  • H315: Causes skin irritation.
  • H319: Causes serious eye irritation.

What is the melting point of NBT?

The melting point is 189°C .

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